genus sphingomonas  (DSMZ)

Journal: Life Metabolism

Article Title: Abnormal adipose tissue-derived microbes drive metabolic disorder and exacerbate postnatal growth retardation in piglet

doi: 10.1093/lifemeta/load052

Figure Lengend Snippet: Identification of a genus in alphaproteobacteria class ( Sphingomonas ) in porcine adipose tissue. (a) Diagram of selection strategy for a targeted microbe. (b) Top 50 overlapped genera in the four tissues. (c and d) Top 20 overlapped genera in the blood (c) and sWAT (d). (e and f) Relative abundance of genera from Proteobacteria in the blood (e) and sWAT (f). (g) Relative abundance of Sphingomonas in sWAT, aWAT, blood, and liver. (h) Workflow of bacteria isolation from adipose tissue. (i) Representative culture plate in the Ctrl and PGR groups. PBS is the negative control. Yellow arrows show the colonies. (j) Quantification of cultivated bacteria in the Ctrl and PGR groups. The statistical significance is denoted as * P < 0.05, ** P < 0.01.

Article Snippet: To test whether bacteria from the Sphingomonas genus play a role in inducing PGR in piglets, one isolate of Sphingomonas , S. paucimobilis (ATCC 29837), was cultured for further study.

Techniques: Selection, Bacteria, Isolation, Negative Control

Journal: Life Metabolism

Article Title: Abnormal adipose tissue-derived microbes drive metabolic disorder and exacerbate postnatal growth retardation in piglet

doi: 10.1093/lifemeta/load052

Figure Lengend Snippet: Gavage of Sphingomonas induces the formation of PGR. (a) Diagram of the experiment design. (b and c) Initial BW (b) and cumulative BW (c) of Saline and Sphingomonas- gaveged piglets. (d–g) Index of bone morphology: Conn. Dens. tibias (d), stiffness (e), load at break (f), and bone mineral content (g) of Saline and Sphingomonas -gavaged groups. (h–k) Levels of leptin (h), adiponectin (i), insulin (j), and HOMA-IR (k) in Saline and Sphingomonas piglets. (l) mRNA levels of genes related to inflammation ( TNFα , IL-1β , and IL-10 ), adipogenesis ( PPARγ , C/EBPα , AdipoQ , and FABP4 ), lipolysis ( HSL and ATGL ), and glucose uptake ( GLUT4 ) in sWAT assessed by qPCR. (m) CARD-FISH on adipose tissue section from Saline and Sphingomonas piglets: Alphaproteobacteria and DAPI staining. Magnification is 100. (n) Positive dot number per section for Alphaproteobacteria by CARD-FISH. (o) Relative abundance of Sphingomonas detected by qPCR. The statistical significance is denoted as: * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: To test whether bacteria from the Sphingomonas genus play a role in inducing PGR in piglets, one isolate of Sphingomonas , S. paucimobilis (ATCC 29837), was cultured for further study.

Techniques: Saline, Staining

Journal: Life Metabolism

Article Title: Abnormal adipose tissue-derived microbes drive metabolic disorder and exacerbate postnatal growth retardation in piglet

doi: 10.1093/lifemeta/load052

Figure Lengend Snippet: Effects of Sphingomonas on metabolism and weight loss under acute stress in the mice. (a) Diagram of Sphingomonas gavage experiment. Abx: antibiotic cocktail. (b) Cumulative BW of PBS and Sphingomonas- gavaged mice. (c–e) Oxygen volume (c), carbon dioxide volume (d), EE (e) of PBS and Sphingomonas -gavaged mice. (f–h) Glucose response curve of OGTT (f), AUC of OGTT (g), and glucose response curve of ipGTT for PBS and Sphingomonas -gavaged mice (h). (i) Fecal energy content of PBS and Sphingomonas -gavaged mice. (j) Small intestine and colon length of PBS and Sphingomonas -gavaged mice. (k and l) Weight (k) and ratio to BW (l) of TA muscle. (m and n) Diagram of dexamethasone treatment in PBS and Sphingomonas -gavaged mice (m) and BW changes (n). Dex: dexamethasone injection. The statistical significance is denoted as: * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: To test whether bacteria from the Sphingomonas genus play a role in inducing PGR in piglets, one isolate of Sphingomonas , S. paucimobilis (ATCC 29837), was cultured for further study.

Techniques: Injection

Journal: Life Metabolism

Article Title: Abnormal adipose tissue-derived microbes drive metabolic disorder and exacerbate postnatal growth retardation in piglet

doi: 10.1093/lifemeta/load052

Figure Lengend Snippet: Effects of PE administration on growth performance and metabolic index of piglets. (a) Diagram of the experiment design. (b) BW of PGR + Saline and PGR + PE piglets. (c) BW gain of PGR + Saline and PGR + PE piglets. (d–h) Femur length (d), Conn. Dens. tibias (e), load at break (f), flexural rigidity (g), and bone mineral content (h) of PGR + Saline and PGR + PE piglets. (i–m) Levels of leptin (i), adiponectin (j), insulin (k), HOMA-IR (l), and glucose (m) in the serum of PGR + Saline and PGR + PE piglets. (n) mRNA levels of genes related to inflammation ( TNFα, IL-1β , and IL-10 ), adipogenesis ( PPARγ , C/EBPα , AdipoQ , and FABP4 ), lipolysis ( HSL and ATGL ), and glucose uptake ( GLUT4 ) in sWAT assessed by qPCR. (o) Relative abundance of Sphingomonas detected by qPCR. The statistical significance is denoted as: * P < 0.05, ** P < 0.01.

Article Snippet: To test whether bacteria from the Sphingomonas genus play a role in inducing PGR in piglets, one isolate of Sphingomonas , S. paucimobilis (ATCC 29837), was cultured for further study.

Techniques: Saline

Journal: Polymers

Article Title: Biopolymers Produced by Sphingomonas Strains and Their Potential Applications in Petroleum Production

doi: 10.3390/polym14091920

Figure Lengend Snippet: Sphingomonas strains and corresponding sphingans.

Article Snippet: Many strains of the genus Sphingomonas have also been reported to produce unusual types of sphingans, such as Sphingomonas sp. CS101 [ ], Sphingomonas sp. R1 [ ], Sphingomonas sp. WG [ ], and Sphingomonas capsulata ATCC 14666 [ ].

Techniques: Control